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KMID : 0357920000340090642
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Korean Journal of Pathology
2000 Volume.34 No. 9 p.642 ~ p.651
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Expression of Transforming Growth Factor-β1 in Cyclosporine-Induced Nephropathy in Rats
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Kang Yu-Na
Park Kwan-Kyu
Hwang Mee-Yul
Kwon Kun-Young
Lee Sang-Sook
Chang Eun-Sook
Kim Hyun-Chul
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Abstract
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Cyclosporine nephropathy was induced by intraperitoneal injection of cyclosporine 25 mg/kg in Sprague-Dawley rats daily for 1, 4, 8, and 12 weeks to clarify the relationship between cyclosporine nephropathy and the expression of TGF-β1 with extracellular matrix deposition. On light microscopic examination, the kidneys in the 12 week cyclosporine-treated rats showed focal or striped fibrosis, vacuolization of tubular cells, and injury of endothelial cells. Immunohistochemically, TGF-β1 protein was strongly expressed in the cyclosporine-treated rat kidneys, especially in the glomerular endothelial cells, interstitial endothelial cells, tubular epithelial cells, and parietal cells in the Bowman’s capsule of the glomerulus as well as the periglomerular arterioles. The amount of TGF-β1 expression was correlated with the morphological change in the cyclosporine-treated rats. Extracellular matrix, such as fibronectin and collagen IV, was also expressed in the endothelial cells of the glomerulus and the interstitium. It can be concluded, therefore that TGF-β1 protein is probably involved in the early stage of fibrogenesis in cyclosporine nephropathy. It can be postulated that cyclosporine nephropathy results from the accumulation of extracellular matrix associated with the increase of TGF-β1 transcription. Therefore, these results could be used in reducing fibrosis in cyclosporine nephropathy.
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KEYWORD
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Cyclosporine nephropathy, Fibrogenesis, TGF-β1 protein, Extracellular matrix
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